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Dr. Luz Gonzalez de Bashan and Dr. Yoav Bashan
Genetic manipulation and molecular detection and monitoring
of plant growth-promoting bacteria (Basic studies)
Version January 2010
Goals: Genetically manipulate Plant Growth-Promoting Bacteria (PGPB);
production of Azospirillum strains with reporter genes (Green Fluorescent Protein). Detection of PGPB by molecular
technique such as Fluorescense in situ Hybridization (FISH) and monitoring microbial populations using Denaturing
Gradient Gel Electrophoresis (DGGE).
For More PDF files of earlier papers go to: Environmental Microbiology
Website.
External collaborators
Dr. Alberto
Mendoza, Centro de Biotecnología Genómica (CBG), Reynosa, Tamaulipas,
México
Prof. Raina Maier, Soil, Water and Environmental Science,
University of Arizona, Arizona, USA
Most relevant publications
Rodriguez, H., Fraga, T. and Bashan, Y.
2006. Genetics of phosphate solubilization and potential applications for improving plant growth-promoting bacteria.
Plant and Soil 287: 15 - 21.
Rodriguez, H., Mendoza, A., Cruz, M.A., Holguin, G., Glick B.R., and Bashan, Y. 2006.
Pleiotropic physiological effects in the plant growth-promoting bacterium Azospirillum brasilense following chromosomal labeling in the clpX gene.
FEMS Microbiology Ecology 57: 217-225.
Bacilio, M., Rodriguez H., Moreno, M., Hernandez,
J.-P., and Bashan Y. 2004. Mitigation of salt stress in wheat seedlings by a gfp-tagged
Azospirillum lipoferum. Biology and
Fertility of Soils 40: 188-193.
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