Dr. Yoav Bashan, Dr. Luz de Bashan,
Dr. Blanca Estela Romero Lopez
and M. Sc. Juan-Pablo Hernandez
Microalge-bacteria interaction (Basic studies)
Version January 2016
Goal: Develop basic research on eukaryote-prokaryote
interactions using a unique combinations of microalgae and microalgae growth-promoting bacteria.
For More PDF files of earlier papers go to: Environmental Microbiology
Website.
External Collaborators
- Dr. Mike Cohen,
Sonoma State University,California, USA
- Dr. Volker Huss,
University of Erlangen-Nurnberg,Germany
- Dr. Brad Bebout,
NASA-Ames,California, USA
- Dr. Leslie Prufert-Bebout,
NASA-Ames,California, USA
- Dr. Xavier Myali,
Lawrence Livermore National Laboratory, California, USA
- Dr. Peter Weber,
Lawrence Livermore National Laboratory, California, USA
- Dra. Gracia Alicia Gómez Anduro,
Center for Biological Research of the Northwest,,La Paz, Mexico
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The history of how the microalgae-plant growth promoting bacteria model was born
click on the imagen
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Cell-cell interaction of the microalgae Chlorella and the bacterium Azospirillum.
A. Scanning Electron Microscopy. B. Showing Carbon enrichment of the same interaction by NanoSIMS
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Transmission electron microscopy of the plant growth-promoting
bacterium Azospirillum brasilense (Az) sharing the
same cavity (c) within alginate beads (Al) with the microalga
Chlorella vulgaris (ch). This unique preparation is
used for wastewater treatment.
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Microalgae used in the nutrient removal from wastewaters.
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Microalgae and the bacterium
A. brasilense
coimmobilized in alginate beads.
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The microalgae and bacteria
coimmobilized in alginate
beads are cultivated
in semicontinuos
systems in a fermentor.
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Most relevant publications in recent years:
- de-Bashan, L. E., Hernandez, J. P., and Bashan, Y. 2015 Interaction of Azospirillum spp.
with Microalgae: A Basic Eukaryotic–Prokaryotic Model and Its Biotechnological Applications. In:
Handbook for Azospirillum (pp. 367-388). Springer International Publishing
- Meza, B., de-Bashan, L.E., and Bashan, Y. 2015. Involvement of indole-3-acetic acid produced by Azospirillum brasilense in accumulating
intracellular ammonium in Chlorella vulgaris.
Research in Microbiology 166: 72-83
- Meza, B., de-Bashan, L. E., Hernandez, J. P., and Bashan, Y. 2015 Accumulation of intra-cellular polyphosphate in Chlorella vulgaris cells is
related to indole-3-acetic acid produced by Azospirillum brasilense.
Research in Microbiology 166: 399-407
- Leyva, L.A., Bashan Y., and de-Bashan, L.E. 2015. Activity
of acetyl-CoA carboxylase is not directly linked to accumulation of lipids when Chlorella vulgaris is co-immobilised with
Azospirillum brasilense in alginate under autotrophic and heterotrophic conditions.
Annals of Microbiology 65: 339-349
- Leyva, L.A., Bashan Y., Mendoza, A., and
de-Bashan, L.E. 2014. Accumulation of fatty acids in Chlorella vulgaris under heterotrophic conditions in relation to activity of
acetyl-CoA carboxylase, temperature, and co-immobilization with Azospirillum brasilense.
Naturwissenschaften 101:819–830
- Choix, F.J., Bashan, Y., Mendoza, A., and de-Bashan, L.E. 2014. Enhanced activity of ADP glucose pyrophosphorylase and formation of
starch induced by Azospirillum brasilense in Chlorella vulgaris.
Journal of Biotechnology 177: 22-34
- Leyva, L.A., Bashan Y., and de-Bashan, L.E. 2014. Activity of acetyl-CoA carboxylase is not directly linked to accumulation of lipids when
Chlorella vulgaris is co-immobilised with Azospirillum brasilense in alginate under autotrophic and heterotrophic conditions.
Annals of Microbiology (in press) DOI 10.1007/s 13213-014-0866-3
- Lopez, B.R., Bashan, Y., Trejo, A., and
de-Bashan, L.E. 2013. Amendment of degraded desert soil with wastewater debris containing immobilized Chlorella sorokiniana and
Azospirillum brasilense significantly modifies soil bacterial community structure, diversity, and richness.
Biology and Fertility of Soils 49: 1053-1063
- Choix, F. J., de-Bashan, L.E. and Bashan, Y. 2012
Enhanced accumulation of starch and total carbohydrates in alginate-immobilized Chlorella spp. induced by
Azospirillum brasilense: I. Autotrophic conditions.
Enzyme and Microbial Technology 51: 294–299
- Choix, F. J., de-Bashan, L.E. and Bashan, Y. 2012
Enhanced accumulation of starch and total carbohydrates in alginate-immobilized Chlorella spp. induced by
Azospirillum brasilense: II. Heterotrophic conditions.
Enzyme and Microbial Technology 51: 300–309
- Trejo, A., de-Bashan, L. E., Hartmann, A., Hernandez, J.P.,
Rothballer, M., Schmid, M. and Bashan, Y. 2012. Recycling waste debris of immobilized microalgae and plant growth-promoting
bacteria from wastewater treatment as a resource to improve fertility of eroded
desert soil. Environmental and Experimental Botany 75 : 65-73
- Covarrubias, S.A., de-Bashan, L.E., Moreno, M., and
Bashan, Y. 2012. Alginate beads provide a beneficial physical barrier against native microorganisms in wastewater treated with immobilized bacteria and
microalgae.
Applied Microbiology and Biotechnology 93: 2669-2680
The definitive version is available electronically on SpringerLink:
http://www.springerlink.com/openurl.asp?genre=article&id=doi:10.1007/s00253-011-3585-8
- Perez-Garcia, O., Escalante, F.M.E., de-Bashan L.E., and Bashan, Y. 2011. Heterotrophic cultures of microalgae: Metabolism and potential products.
Water Research 45: 11-36.
The definitive version is available at: http://www.elsevier.com
- de-Bashan, L.E., Schmid, M., Rothballer, M., Hartmann, A., and Bashan Y., 2011. Cell-cell interaction in the eukaryote-prokaryote model using the microalgae Chlorella vulgaris and the bacterium Azospirillum brasilense immobilized in polymer beads. Journal of Phycology 47:1350-1359
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Hernandez, J.-P., de-Bashana,L.E., Rodriguez, D. J., Rodriguez, Y. and Bashan, Y. 2009 Growth promotion of the freshwater
microalga Chlorella vulgaris by the nitrogen-fixing, plant growth-promoting bacterium Bacillus pumilus
from arid zone soils. European Journal of Soil Biology 45: 88–93
Original version is available at: http://www.elsevier.com/locate/ejsobi
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de-Bashan, L.E., and Bashan Y. 2008. Joint immobilization of plant growth-promoting bacteria and green microalgae
in alginate beads as an experimental model for studying plant-bacterium interactions.
Applied and Environmental Microbiology 74: 6797–6802.
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de-Bashan, L.E., Antoun, H., and Bashan Y. 2008. Involvement of indole-3-acetic-acid produced by the growth-promoting
bacterium Azospirillum spp. in promoting growth of Chlorella vulgaris.
Journal of Phycology 44: 938–947.
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de-Bashan, L.E., Magallon, P., Antoun, H., and Bashan Y. 2008. Role of glutamate dehydrogenase and
glutamine synthetase in Chlorella vulgaris during assimilation of ammonium when jointly
immobilized with the microalgae-growth-promoting bacterium Azospirillum brasilense.
Journal of Phycology 44: 1188–1196.
- de-Bashan, L.E., Antoun H., and Bashan, Y. 2005. Cultivation factors and population size control uptake of nitrogen by the microalgae Chlorella vulgaris when interacting with the microalgae growth-promoting baterium Azospirillum brasilense.
FEMS Microbiology Ecology 54: 197-203
- de-Bashan L.E., Hernandez
J.-P., Morey, T., and Bashan, Y. 2004. Microalgae growth-promoting
bacteria as "helpers" for microalgae: a novel approach for
removing ammonium and phosphorus for municipal wastewater.
Water Research 38:466-474.
- de-Bashan, L. E. and Bashan, Y. 2003. Bacterias promotoras de crecimiento de
microalgas: una nueva aproximación en el tratamiento de aguas residuales
(Microalgae growth-proomoting bacteria: a novel approach in wastewater
treatment).
Revista
Colombiana de Biotecnología 5: 85-90.
- de-Bashan, L.E., Bashan, Y., Moreno, M., Lebsky, V.K., and Bustillos,J.J.
2002. Increased pigment and lipid content, lipid variety, and cell and population
size of the microalgae Chlorella spp. when co-immobilized in alginate
beads with the microalgae-growth-promoting bacterium Azospirillum brasilense
.
Canadian Journal of Microbiology 48: 514-521.
- Lebsky, V.K., Gonzalez-Bashan, L.E., and Bashan, Y. 2001. Ultrastructure
of coimmobilization of the microalga Chlorella vulgaris with
the plant growth-promoting bacterium Azospirillum brasilense
and with its natural associative bacterium Phyllobacterium
myrsinacearum in alginate beads.
Canadian Journal of Microbiology 47 : 1-8.

Image of Confocal Laser Microscopy of clusters of the microalgae Chlorella vulgaris with the microalgae growth-promoting bacterium Azospirillum brasilense after interaction and dissolving the alginates beads where both were initially immobilized. Arrows marking red spots indicating the bacteria. The microalgae (larger circles) have different fluorescent colors.
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Image of Confocal Laser Microscopy of clusters of the microalgae Chlorella vulgaris with the microalgae growth-promoting bacterium Azospirillum brasilense after interaction and dissolving the alginates beads where both were initially immobilized. Arrows marking small yellow spots indicating the bacteria. The microalgae (larger circles) have different fluorescent colors.
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Related pages:
- Device for producing large-sized polymer beads
- A method for automated fast production of
large-sized polymer beads (2–4 mm)
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