CIBNOR, Yoav Bashan, Microalga-Bacteria interaction

Dr. Yoav Bashan, Dr. Luz Gonzalez de Bashan
and M. Sc. Juan-Pablo Hernandez

Microalge-bacteria interaction
(Basic studies)

Version January 2012

Goal: Develop basic research on eukaryote-prokaryote interactions using a unique combinations of microalgae and microalgae growth-promoting bacteria.

For More PDF files of earlier papers go to: Environmental Microbiology Website.

For Spanish speakers (español)

For English speakers

External Collaborators

Dr. Drora Kaplan, Ben Gurion University, Israel

Dr. Zvi Hacohen, Ben Gurion University, Israel

Dr. Mike Cohen, Sonoma State University,California, USA

Dr. Naresh Singhal, University of Auckland, New Zealand

The history of how the microalgae-plant growth promoting bacteria model
was born
click on the imagen

Transmission electron microscopy of the plant growth-promoting bacterium Azospirillum brasilense (Az) sharing the same cavity (c) within alginate beads (Al) with the microalga Chlorella vulgaris (ch). This unique preparation is used for wastewater treatment.

Microalgae used in the nutrient removal from wastewaters.

Microalgae and the bacterium A. brasilense coimmobilized in alginate beads.

The microalgae and bacteria coimmobilized in alginate beads are cultivated in semicontinuos systems in a fermentor.

Most relevant publications in recent years:

Perez-Garcia, O., Escalante, F.M.E., de-Bashan L.E., and Bashan, Y. 2011. Heterotrophic cultures of microalgae: Metabolism and potential products. Water Research 45: 11-36. The definitive version is available at: http://www.elsevier.com

de-Bashan, L.E., Schmid, M., Rothballer, M., Hartmann, A., and Bashan Y., 2011. Cell-cell interaction in the eukaryote-prokaryote model using the microalgae Chlorella vulgaris and the bacterium Azospirillum brasilense immobilized in polymer beads. Journal of Phycology 47:1350-1359

Hernandez, J.-P., de-Bashana,L.E., Rodriguez, D. J., Rodriguez, Y. and Bashan, Y. 2009 Growth promotion of the freshwater microalga Chlorella vulgaris by the nitrogen-fixing, plant growth-promoting bacterium Bacillus pumilus from arid zone soils. European Journal of Soil Biology 45: 88–93 Original version is available at: http://www.elsevier.com/locate/ejsobi

de-Bashan, L.E., and Bashan Y. 2008. Joint immobilization of plant growth-promoting bacteria and green microalgae in alginate beads as an experimental model for studying plant-bacterium interactions. Applied and Environmental Microbiology 74: 6797–6802.

de-Bashan, L.E., Antoun, H., and Bashan Y. 2008. Involvement of indole-3-acetic-acid produced by the growth-promoting bacterium Azospirillum spp. in promoting growth of Chlorella vulgaris. Journal of Phycology 44: 938–947.

de-Bashan, L.E., Magallon, P., Antoun, H., and Bashan Y. 2008. Role of glutamate dehydrogenase and glutamine synthetase in Chlorella vulgaris during assimilation of ammonium when jointly immobilized with the microalgae-growth-promoting bacterium Azospirillum brasilense. Journal of Phycology 44: 1188–1196.

de-Bashan, L.E., Antoun H., and Bashan, Y. 2005. Cultivation factors and population size control uptake of nitrogen by the microalgae Chlorella vulgaris when interacting with the microalgae growth-promoting baterium Azospirillum brasilense. FEMS Microbiology Ecology 54: 197-203

de-Bashan L.E., Hernandez J.-P., Morey, T., and Bashan, Y. 2004. Microalgae growth-promoting bacteria as "helpers" for microalgae: a novel approach for removing ammonium and phosphorus for municipal wastewater. Water Research 38:466-474.

de-Bashan, L. E. and Bashan, Y. 2003. Bacterias promotoras de crecimiento de microalgas: una nueva aproximación en el tratamiento de aguas residuales (Microalgae growth-proomoting bacteria: a novel approach in wastewater treatment). Revista Colombiana de Biotecnología 5: 85-90.

de-Bashan, L.E., Bashan, Y., Moreno, M., Lebsky, V.K., and Bustillos,J.J. 2002. Increased pigment and lipid content, lipid variety, and cell and population size of the microalgae Chlorella spp. when co-immobilized in alginate beads with the microalgae-growth-promoting bacterium Azospirillum brasilense . Canadian Journal of Microbiology 48: 514-521.

Lebsky, V.K., Gonzalez-Bashan, L.E., and Bashan, Y. 2001. Ultrastructure of coimmobilization of the microalga Chlorella vulgaris with the plant growth-promoting bacterium Azospirillum brasilense and with its natural associative bacterium Phyllobacterium myrsinacearum in alginate beads. Canadian Journal of Microbiology 47 : 1-8.

Image of Confocal Laser Microscopy of clusters of the microalgae Chlorella vulgaris with the microalgae growth-promoting bacterium Azospirillum brasilense after interaction and dissolving the alginates beads where both were initially immobilized. Arrows marking red spots indicating the bacteria. The microalgae (larger circles) have different fluorescent colors.

Image of Confocal Laser Microscopy of clusters of the microalgae Chlorella vulgaris with the microalgae growth-promoting bacterium Azospirillum brasilense after interaction and dissolving the alginates beads where both were initially immobilized. Arrows marking small yellow spots indicating the bacteria. The microalgae (larger circles) have different fluorescent colors.

Device for producing large-sized polymer beads

A method for automated fast production of large-sized polymer beads (2–4 mm)