Dr. Yoav Bashan, Dr. Luz de Bashan,
Dr. Blanca Estela Romero Lopez
y M.C. Juan Pablo Hernández

Interaccion microalga bacteria
(estudios básicos)

Versión Enere 2016

Objetivo: Desarrollar conceptos basicos en las interacciones de eucariotes y procariotes usando una combinacion unica de microalga y bacteria promotora de crecimiento en plantas.

Para obtener los PDF de publicaciones anteriores ir al sitio de Microbiología Ambiental.

Colaboraciones externas:

beads, microalgae, bacteria, azospirillum, chlorella
Historia de como inicio el modelo microalga-Bacteria Promotora de Crecimiento
en Plntas
click en la imagen/
nanosims chlorella azospirillum nanosims chlorella azospirillum

Interacción célula-célula de las microalgas Chlorella y la bacteria Azospirillum. A. Microscopio Electronico de Barrido. B. Muestra el enriquecimiento de Carbono de la misma interacción usando NanoSIMS

Micrografía de microscopía electrónica de la bacteria promotora del crecimiento en plantas Azospirillum brasilense (Az) y de la microalga Chlorella vulgaris (ch), compartiendo la misma cavidad (c) dentro de una esfera de alginato (Al). Esta combinación es usada para el tratamiento de aguas residuales.

Microalgas usadas en la eliminación de nutrientes de aguas residuales.

Esferas de alginato, con las microalgas y bacterias.

Las microalgas y bacterias coinmovilizadas en esferas de alginato son cultivadas en sistemas semicontínuos en un quimiostato.

Publicaciones más relevantes:

  • de-Bashan, L. E., Hernandez, J. P., and Bashan, Y. 2015 Interaction of Azospirillum spp. with Microalgae: A Basic Eukaryotic–Prokaryotic Model and Its Biotechnological Applications. In: Handbook for Azospirillum (pp. 367-388). Springer International Publishing

  • Meza, B., de-Bashan, L.E., and Bashan, Y. 2015. Involvement of indole-3-acetic acid produced by Azospirillum brasilense in accumulating intracellular ammonium in Chlorella vulgaris. Research in Microbiology 166: 72-83

  • Meza, B., de-Bashan, L. E., Hernandez, J. P., and Bashan, Y. 2015 Accumulation of intra-cellular polyphosphate in Chlorella vulgaris cells is related to indole-3-acetic acid produced by Azospirillum brasilense. Research in Microbiology 166: 399-407

  • Leyva, L.A., Bashan Y., and de-Bashan, L.E. 2015. Activity of acetyl-CoA carboxylase is not directly linked to accumulation of lipids when Chlorella vulgaris is co-immobilised with Azospirillum brasilense in alginate under autotrophic and heterotrophic conditions. Annals of Microbiology 65: 339-349

  • Leyva, L.A., Bashan Y., Mendoza, A., and de-Bashan, L.E. 2014. Accumulation of fatty acids in Chlorella vulgaris under heterotrophic conditions in relation to activity of acetyl-CoA carboxylase, temperature, and co-immobilization with Azospirillum brasilense. Naturwissenschaften 101:819–830

  • Leyva, L.A., Bashan Y., Mendoza, A., and de-Bashan, L.E. 2014. Accumulation of fatty acids in Chlorella vulgaris under heterotrophic conditions in relation to activity of acetyl-CoA carboxylase, temperature, and co-immobilization with Azospirillum brasilense. Naturwissenschaften 101:819–830

  • Choix, F.J., Bashan, Y., Mendoza, A., and de-Bashan, L.E. 2014. Enhanced activity of ADP glucose pyrophosphorylase and formation of starch induced by Azospirillum brasilense in Chlorella vulgaris. Journal of Biotechnology 177: 22-34

  • Leyva, L.A., Bashan Y., and de-Bashan, L.E. 2014. Activity of acetyl-CoA carboxylase is not directly linked to accumulation of lipids when Chlorella vulgaris is co-immobilised with Azospirillum brasilense in alginate under autotrophic and heterotrophic conditions. Annals of Microbiology (in press) DOI 10.1007/s 13213-014-0866-3

  • Lopez, B.R., Bashan, Y., Trejo, A., and de-Bashan, L.E. 2013. Amendment of degraded desert soil with wastewater debris containing immobilized Chlorella sorokiniana and Azospirillum brasilense significantly modifies soil bacterial community structure, diversity, and richness. Biology and Fertility of Soils 49: 1053-1063

  • Choix, F. J., de-Bashan, L.E. and Bashan, Y. 2012 Enhanced accumulation of starch and total carbohydrates in alginate-immobilized Chlorella spp. induced by Azospirillum brasilense: I. Autotrophic conditions. Enzyme and Microbial Technology 51: 294–299

  • Choix, F. J., de-Bashan, L.E. and Bashan, Y. 2012 Enhanced accumulation of starch and total carbohydrates in alginate-immobilized Chlorella spp. induced by Azospirillum brasilense: II. Heterotrophic conditions. Enzyme and Microbial Technology 51: 300–309

  • Trejo, A., de-Bashan, L. E., Hartmann, A., Hernandez, J.P., Rothballer, M., Schmid, M. and Bashan, Y. 2012. Recycling waste debris of immobilized microalgae and plant growth-promoting bacteria from wastewater treatment as a resource to improve fertility of eroded desert soil. Environmental and Experimental Botany 75 : 65-73

  • Covarrubias, S.A., de-Bashan, L.E., Moreno, M., and Bashan, Y. 2012. Alginate beads provide a beneficial physical barrier against native microorganisms in wastewater treated with immobilized bacteria and microalgae. Applied Microbiology and Biotechnology 93: 2669-2680
    The definitive version is available electronically on SpringerLink: http://www.springerlink.com/openurl.asp?genre=article&id=doi:10.1007/s00253-011-3585-8

  • Perez-Garcia, O., Escalante, F.M.E., de-Bashan L.E., and Bashan, Y. 2011. Heterotrophic cultures of microalgae: Metabolism and potential products. Water Research 45: 11-36. The definitive version is available at: http://www.elsevier.com

  • de-Bashan, L.E., Schmid, M., Rothballer, M., Hartmann, A., and Bashan Y., 2011. Cell-cell interaction in the eukaryote-prokaryote model using the microalgae Chlorella vulgaris and the bacterium Azospirillum brasilense immobilized in polymer beads. Journal of Phycology 47:1350-1359

  • Hernandez, J.-P., de-Bashana,L.E., Rodriguez, D. J., Rodriguez, Y. and Bashan, Y. 2009 Growth promotion of the freshwater microalga Chlorella vulgaris by the nitrogen-fixing, plant growth-promoting bacterium Bacillus pumilus from arid zone soils. European Journal of Soil Biology 45: 88–93 Original version is available at: http://www.elsevier.com/locate/ejsobi

  • de-Bashan, L.E., and Bashan Y. 2008. Joint immobilization of plant growth-promoting bacteria and green microalgae in alginate beads as an experimental model for studying plant-bacterium interactions. Applied and Environmental Microbiology 74: 6797–6802.

  • de-Bashan, L.E., Antoun, H., and Bashan Y. 2008. Involvement of indole-3-acetic-acid produced by the growth-promoting bacterium Azospirillum spp. in promoting growth of Chlorella vulgaris. Journal of Phycology 44: 938–947.

  • de-Bashan, L.E., Magallon, P., Antoun, H., and Bashan Y. 2008. Role of glutamate dehydrogenase and glutamine synthetase in Chlorella vulgaris during assimilation of ammonium when jointly immobilized with the microalgae-growth-promoting bacterium Azospirillum brasilense. Journal of Phycology 44: 1188–1196.

  • de-Bashan, L.E., Antoun H., and Bashan, Y. 2005. Cultivation factors and population size control uptake of nitrogen by the microalgae Chlorella vulgaris when interacting with the microalgae growth-promoting baterium Azospirillum brasilense. FEMS Microbiology Ecology 54: 197-203

  • de-Bashan L.E., Hernandez J.-P., Morey, T., and Bashan, Y. 2004. Microalgae growth-promoting bacteria as "helpers" for microalgae: a novel approach for removing ammonium and phosphorus for municipal wastewater. Water Research 38:466-474.
  • de-Bashan, L. E. and Bashan, Y. 2003. Bacterias promotoras de crecimiento de microalgas: una nueva aproximación en el tratamiento de aguas residuales (Microalgae growth-proomoting bacteria: a novel approach in wastewater treatment). Revista Colombiana de Biotecnología 5: 85-90.

  • de-Bashan, L.E., Bashan, Y., Moreno, M., Lebsky, V.K., and Bustillos,J.J. 2002. Increased pigment and lipid content, lipid variety, and cell and population size of the microalgae Chlorella spp. when co-immobilized in alginate beads with the microalgae-growth-promoting bacterium Azospirillum brasilense . Canadian Journal of Microbiology 48: 514-521.
  • Lebsky, V.K., Gonzalez-Bashan, L.E., and Bashan, Y. 2001. Ultrastructure of coimmobilization of the microalga Chlorella vulgaris with the plant growth-promoting bacterium Azospirillum brasilense and with its natural associative bacterium Phyllobacterium myrsinacearum in alginate beads. Canadian Journal of Microbiology 47 : 1-8.


  • Imagnes en Microscópio Laser Confocal de los conglomerados de la microalga Chlorella vulgaris con la Bacteria Promotora de Crecimiento en Microalgas Azospirillum brasilense despues de la interacción y la disolución de las esferas de alginato, donde fueron inmovilizadas inicialmente. Las flechas muestran los puntos rojos indicando a la bacteria. La microalga (circulos grandes) tiene diferentes colores fluorescentes.

    Imagnes en Microscópio Laser Confocal de los conglomerados de la microalga Chlorella vulgaris con la Bacteria Promotora de Crecimiento en Microalgas Azospirillum brasilense despues de la interacción y la disolución de las esferas de alginato, donde fueron inmovilizadas inicialmente. Las flechas muestran los puntos amarillos indicando a la bacteria. La microalga (circulos grandes) tiene diferentes colores fluorescentes.


    Pagínas relacionadas:

  • Equipo para la producción de esferas de alginato (en Ingles)

  • Método para la automática producción de esferas de polimeros (2-4 mm) (en Ingles)



    Página personal de Yoav Bashan         Página personal de Luz González de Bashan

    Página personal de Blanca Estela Romero lópez        Página personal de Luz González de Bashan

    Comentarios sobre esta página: Juan Pablo Hernandez

    Volver a la sección     Grupo de Microbiología Ambiental