Dr. Yoav Bashan, Dr. Luz de Bashan, Dr. Blanca Estela Romero Lopez
y M.C. Juan Pablo Hernández
Interaccion microalga bacteria (estudios básicos)
Versión Enere 2016
Objetivo: Desarrollar conceptos
basicos en las interacciones de eucariotes y procariotes usando una combinacion unica de microalga y bacteria promotora de crecimiento en plantas.
Para obtener los PDF de publicaciones anteriores ir al sitio de Microbiología Ambiental.
Colaboraciones externas:
- Dr. Mike Cohen,
Sonoma State University,California, USA
- Dr. Volker Huss,
University of Erlangen-Nurnberg,Germany
- Dr. Brad Bebout,
NASA-Ames,California, USA
- Dr. Leslie Prufert-Bebout,
NASA-Ames,California, USA
- Dr. Xavier Myali,
Lawrence Livermore National Laboratory, California, USA
- Dr. Peter Weber,
Lawrence Livermore National Laboratory, California, USA
- Dra. Gracia Alicia Gómez Anduro,
Center for Biological Research of the Northwest,,La Paz, Mexico
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Historia de como inicio el modelo microalga-Bacteria Promotora de Crecimiento en Plntas click en la imagen/
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Interacción célula-célula de las microalgas Chlorella y la bacteria Azospirillum.
A. Microscopio Electronico de Barrido. B. Muestra el enriquecimiento de Carbono de la misma
interacción usando NanoSIMS
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Micrografía de microscopía electrónica
de la bacteria promotora del crecimiento en plantas
Azospirillum brasilense (Az) y de la microalga
Chlorella vulgaris (ch), compartiendo la misma
cavidad (c) dentro de una esfera de alginato (Al). Esta
combinación es usada para el tratamiento de aguas residuales.
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Microalgas usadas en la eliminación de nutrientes
de aguas residuales.
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Esferas de alginato, con las microalgas y bacterias.
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Las microalgas y bacterias coinmovilizadas en esferas
de alginato son cultivadas en sistemas semicontínuos
en un quimiostato.
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Publicaciones más relevantes:
- de-Bashan, L. E., Hernandez, J. P., and Bashan, Y. 2015 Interaction of Azospirillum spp.
with Microalgae: A Basic Eukaryotic–Prokaryotic Model and Its Biotechnological Applications. In:
Handbook for Azospirillum (pp. 367-388). Springer International Publishing
- Meza, B., de-Bashan, L.E., and Bashan, Y. 2015. Involvement of indole-3-acetic acid produced by Azospirillum brasilense in accumulating
intracellular ammonium in Chlorella vulgaris.
Research in Microbiology 166: 72-83
- Meza, B., de-Bashan, L. E., Hernandez, J. P., and Bashan, Y. 2015 Accumulation of intra-cellular polyphosphate in Chlorella vulgaris cells is
related to indole-3-acetic acid produced by Azospirillum brasilense.
Research in Microbiology 166: 399-407
- Leyva, L.A., Bashan Y., and de-Bashan, L.E. 2015. Activity
of acetyl-CoA carboxylase is not directly linked to accumulation of lipids when Chlorella vulgaris is co-immobilised with
Azospirillum brasilense in alginate under autotrophic and heterotrophic conditions.
Annals of Microbiology 65: 339-349
- Leyva, L.A., Bashan Y., Mendoza, A., and
de-Bashan, L.E. 2014. Accumulation of fatty acids in Chlorella vulgaris under heterotrophic conditions in relation to activity of
acetyl-CoA carboxylase, temperature, and co-immobilization with Azospirillum brasilense.
Naturwissenschaften 101:819–830
- Leyva, L.A., Bashan Y., Mendoza, A., and
de-Bashan, L.E. 2014. Accumulation of fatty acids in Chlorella vulgaris under heterotrophic conditions in relation to activity of
acetyl-CoA carboxylase, temperature, and co-immobilization with Azospirillum brasilense.
Naturwissenschaften 101:819–830
- Choix, F.J., Bashan, Y., Mendoza, A., and de-Bashan, L.E. 2014. Enhanced activity of ADP glucose pyrophosphorylase and formation of
starch induced by Azospirillum brasilense in Chlorella vulgaris.
Journal of Biotechnology 177: 22-34
- Leyva, L.A., Bashan Y., and de-Bashan, L.E. 2014. Activity of acetyl-CoA carboxylase is not directly linked to accumulation of lipids when
Chlorella vulgaris is co-immobilised with Azospirillum brasilense in alginate under autotrophic and heterotrophic conditions.
Annals of Microbiology (in press) DOI 10.1007/s 13213-014-0866-3
- Lopez, B.R., Bashan, Y., Trejo, A., and
de-Bashan, L.E. 2013. Amendment of degraded desert soil with wastewater debris containing immobilized Chlorella sorokiniana and
Azospirillum brasilense significantly modifies soil bacterial community structure, diversity, and richness.
Biology and Fertility of Soils 49: 1053-1063
- Choix, F. J., de-Bashan, L.E. and Bashan, Y. 2012
Enhanced accumulation of starch and total carbohydrates in alginate-immobilized Chlorella spp. induced by
Azospirillum brasilense: I. Autotrophic conditions.
Enzyme and Microbial Technology 51: 294–299
- Choix, F. J., de-Bashan, L.E. and Bashan, Y. 2012
Enhanced accumulation of starch and total carbohydrates in alginate-immobilized Chlorella spp. induced by
Azospirillum brasilense: II. Heterotrophic conditions.
Enzyme and Microbial Technology 51: 300–309
- Trejo, A., de-Bashan, L. E., Hartmann, A., Hernandez, J.P.,
Rothballer, M., Schmid, M. and Bashan, Y. 2012. Recycling waste debris of immobilized microalgae and plant growth-promoting
bacteria from wastewater treatment as a resource to improve fertility of eroded
desert soil. Environmental and Experimental Botany 75 : 65-73
- Covarrubias, S.A., de-Bashan, L.E., Moreno, M., and
Bashan, Y. 2012. Alginate beads provide a beneficial physical barrier against native microorganisms in wastewater treated with immobilized bacteria and
microalgae.
Applied Microbiology and Biotechnology 93: 2669-2680
The definitive version is available electronically on SpringerLink:
http://www.springerlink.com/openurl.asp?genre=article&id=doi:10.1007/s00253-011-3585-8
- Perez-Garcia, O., Escalante, F.M.E., de-Bashan L.E., and Bashan, Y. 2011. Heterotrophic cultures of microalgae: Metabolism and potential products.
Water Research 45: 11-36.
The definitive version is available at: http://www.elsevier.com
- de-Bashan, L.E., Schmid, M., Rothballer, M., Hartmann, A., and Bashan Y., 2011. Cell-cell interaction in the eukaryote-prokaryote model using the microalgae Chlorella vulgaris and the bacterium Azospirillum brasilense immobilized in polymer beads. Journal of Phycology 47:1350-1359
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Hernandez, J.-P., de-Bashana,L.E., Rodriguez, D. J., Rodriguez, Y. and Bashan, Y. 2009 Growth promotion of the freshwater
microalga Chlorella vulgaris by the nitrogen-fixing, plant growth-promoting bacterium Bacillus pumilus
from arid zone soils. European Journal of Soil Biology 45: 88–93
Original version is available at: http://www.elsevier.com/locate/ejsobi
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de-Bashan, L.E., and Bashan Y. 2008. Joint immobilization of plant growth-promoting bacteria and green microalgae
in alginate beads as an experimental model for studying plant-bacterium interactions.
Applied and Environmental Microbiology 74: 6797–6802.
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de-Bashan, L.E., Antoun, H., and Bashan Y. 2008. Involvement of indole-3-acetic-acid produced by the growth-promoting
bacterium Azospirillum spp. in promoting growth of Chlorella vulgaris.
Journal of Phycology 44: 938–947.
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de-Bashan, L.E., Magallon, P., Antoun, H., and Bashan Y. 2008. Role of glutamate dehydrogenase and
glutamine synthetase in Chlorella vulgaris during assimilation of ammonium when jointly
immobilized with the microalgae-growth-promoting bacterium Azospirillum brasilense.
Journal of Phycology 44: 1188–1196.
- de-Bashan, L.E., Antoun H., and Bashan, Y. 2005. Cultivation factors and population size control uptake of nitrogen by the microalgae Chlorella vulgaris when interacting with the microalgae growth-promoting baterium Azospirillum brasilense.
FEMS Microbiology Ecology 54: 197-203
- de-Bashan L.E., Hernandez
J.-P., Morey, T., and Bashan, Y. 2004. Microalgae growth-promoting
bacteria as "helpers" for microalgae: a novel approach for
removing ammonium and phosphorus for municipal wastewater.
Water Research 38:466-474.
- de-Bashan, L. E. and Bashan, Y. 2003. Bacterias promotoras de crecimiento de
microalgas: una nueva aproximación en el tratamiento de aguas residuales
(Microalgae growth-proomoting bacteria: a novel approach in wastewater
treatment).
Revista
Colombiana de Biotecnología 5: 85-90.
- de-Bashan, L.E., Bashan, Y., Moreno, M., Lebsky, V.K., and Bustillos,J.J.
2002. Increased pigment and lipid content, lipid variety, and cell and population
size of the microalgae Chlorella spp. when co-immobilized in alginate
beads with the microalgae-growth-promoting bacterium Azospirillum brasilense
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Canadian Journal of Microbiology 48: 514-521.
- Lebsky, V.K., Gonzalez-Bashan, L.E., and Bashan, Y. 2001. Ultrastructure
of coimmobilization of the microalga Chlorella vulgaris with
the plant growth-promoting bacterium Azospirillum brasilense
and with its natural associative bacterium Phyllobacterium
myrsinacearum in alginate beads.
Canadian Journal of Microbiology 47 : 1-8.

Imagnes en Microscópio Laser Confocal de los conglomerados de la microalga Chlorella vulgaris con la Bacteria Promotora de Crecimiento en Microalgas Azospirillum brasilense despues de la interacción y la disolución de las esferas de alginato, donde fueron inmovilizadas inicialmente. Las flechas muestran los puntos rojos indicando a la bacteria. La microalga (circulos grandes) tiene diferentes colores fluorescentes.
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Imagnes en Microscópio Laser Confocal de los conglomerados de la microalga Chlorella vulgaris con la Bacteria Promotora de Crecimiento en Microalgas Azospirillum brasilense despues de la interacción y la disolución de las esferas de alginato, donde fueron inmovilizadas inicialmente. Las flechas muestran los puntos amarillos indicando a la bacteria. La microalga (circulos grandes) tiene diferentes colores fluorescentes.
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Pagínas relacionadas:
- Equipo para la producción de esferas de alginato (en Ingles)
- Método para la automática producción de esferas de polimeros (2-4 mm) (en Ingles)
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