The "Environmental Microbiology" group strives to excel in the research of its graduate students and on "tailor-made" theses. Each of our students is required to publish his/her thesis or main findings in high impact, peer-reviewed scientific journals. "Instant" and "assembly line" theses are therefore, unavailable.

Graduated students (since 1992)

Name: Gerardo Toledo
Degree: M.Sc.
Years: 1993-1995
Graduate school: The Interdiciplinary Center for Marine Sciences, National Polytechnique Institute. La Paz, B.C.S., Mexico.
Thesis: Diazotrophic activity of cyanobacteria associated with the black mangrove (Avicennia germinans).
Publications from thesis:
  1. Toledo, G., Bashan, Y. and Soeldner, A. 1995 Cyanobacteria and black mangroves in Northwestern Mexico: colonization, and diurnal and seasonal nitrogen fixation on aerial roots. Canadian Journal of Microbiology 41: 999-1011.
  2. Toledo, G., Bashan, Y. and Soeldner, A. 1995. In vitro colonization and increase in nitrogen fixation of seedling roots of black mangrove inoculated by a filamentous cyanobacteria. Canadian Journal of Microbiology 41: 1012-1020.
  3. Toledo, G., Rojas, A., and Bashan, Y. 2001. Monitoring of black mangrove restoration with nursery-reared seedlings on an arid coastal lagoon. Hydrobiología 444: 101-109.
Gerardo Toledo
Gerardo Toledo plants a seedling.
Gerardo Toledo
Gerardo Toledo is preparing a destroyed mangrove area for reforestation.
Destroyed mangrove area
Gerardo Toledo and Angel Carrillo preparing a destroyed mangrove area for reforestation.

Name: Gina Holguin
Degree: M.Sc.
Years: 1994-1996
Graduate school: The Interdiciplinary Center for Marine Sciences, National Polytechnique Institute. La Paz, B.C.S., Mexico.
Thesis: Co-culturing of Azospirillum brasilense Cd with the mangrove rhizosphere bacteria Staphylococcus sp. promotes its nitrogen fixation.
Publication from thesis:
  1. Holguin, G. y Bashan, Y. 1996. Nitrogen-fixation by Azospirillum brasilense Cd is promoted when co-cultured with a mangrove rhizosphere bacterium (Staphylococcus sp.) Soil Biology and Biochemistry 28: 1651-1660.

Name: Angel Carrillo
Degree: M.Sc.
Years: 1998-2001
Graduate school: The Center for Biological Research of the Northwest, La Paz, Mexico
Thesis: Factors favoring the establishment of plantlets of the cardon cactus (Pachycereus pringlei) in association with mesquite tree (Prosopis articulata) soil.
Publications from thesis:
  1. Carrillo-Garcia, A., Leon de la Luz, J.-L., Bashan, Y. y Bethlenfalvay, G.J. 1999. Nurse plants, mycorrhizae, and plant establishment in a disturbed area of the Sonoran desert. Restoration Ecology 7: 321-335.
  2. Carrillo-Gracia, A., Bashan, Y., Diaz-Rivera, E., y Bethlenfalvay,G.J. 2000. Effects of resource - island soils, competition, and inoculation with Azospirillum on survival and growth of Pachycereus pringlei, the giant cactus of the Sonoran Desert. Restoration Ecology 8 : 65-73.
  3. Carrillo-Garcia, A., Bashan, Y. y Bethlenfalvay, G.J. 2000. Resource-island soils and the survival of the giant cactus, cardon, of Baja California Sur.Plant and Soil 218: 207-214.
  4. Carrillo, A.E., Li, C.Y., and Bashan, Y. 2002. Increased acidification in the rhizosphere of cactus seedlings induced by Azosirillum brasilense. Naturwissenschaften 89: 428-432.

Name: Juan-Pablo Hernandez
Degree: M.Sc.
Years: 2002-2004
Graduate school: The Interdiciplinary Center for Marine Sciences, National Polytechnique Institute. La Paz, B.C. S., México.
Thesis: Evaluation of microalgae-bacteria system for the removal of nutrients from domestic wastewater.
juan-pablo hernandez

Juan-Pablo Hernandez collects sample of wastewater from the wastewater treatment plant of La Paz, Mexico
Publications from thesis:
  1. de Bashan, L. E.,Hernandez, J. P., Morey, T., and Bashan, Y. 2004. Microalgae growth-promoting bacteria as "helpers" for microalgae: a novel approach for removing ammonium and phosphorus from municipal wastewater. Water Research 38:466-474.
  2. Hernandez, J-P., de-Bashan, L.E., and Bashan, Y. 2006. Starvation enhances phosphorus removal from wastewater by the microalgae Chlorella spp.co-immobilized with Azospirillum brasilense. Enzyme and Microbial Technology 38: 190-198

Silke at work

Silke Fendrich preparing a solution to make the beads for wastewater treatment with microalge.
Name: Silke Fendrich
Degree: Diploma (M.Sc. equivalent) in Environmental Engineering
Years: 2004-2005
Graduate school: Technical University of Hamburg-Harburg, Hamburg, Germany.
Thesis: Examination of Chlorella sorokiniana under arid climatic conditions with regard to its capacity for nutrient removal in domestic wastewater.

Name: Ana Lidia Flores Mireles
Degree: M.Sc.
Years: 2003-2005
Graduate school: The Center for Biological Research of the Northwest. La Paz, Mexico.
Thesis: Cell-cell signaling among denitrifying and nitrogen fixing bacteria, asociated to the rhizosphere of black mangrove Avicennia germinans, through Acyl-Homoserin Lactone (AHL's) molecules.
chlorella

(Left to Right) Ana Flores, Alfonso Davila and Claudia Villicaña taking samples from the mangroves at Balandra lagoon.
Publications from thesis:
  1. Flores-Mireles, A.L., S.C. Winans, and G. Holguín. 2007. Molecular characterization of diazotrophic and denitrifying bacteria associated with mangrove roots. Applied and Environmental Microbiology 73: 7308–7321.

Name: Luis Alonso Leyva
Degree: M.Sc.
Years: 2003-2006
Graduate school: The Center for Biological Research of the Northwest. La Paz, Mexico.
Thesis: Effect of gluconic acid on its metabolic enzymes in Azospirillum brasilense associated with mesquite tree roots.
Publication from thesis:
  1. Leyva L.A. and Bashan, Y. 2008. Activity of two catabolic enzymes of the phosphogluconate pathway in mesquite roots inoculated with Azospirillum brasilense Cd. Plant Physiology and Biochemistry 46 : 898-904

Name: Maria Claudia Villicaña Torres
Degree: M. Sc.
Years: 2004-2007
Graduate school: The Center for Biological Research of the Northwest. La Paz, Mexico.
Thesis: Effect of acyl homoserine lactones on nitrogen fixation and inorganic phosphate solubilization in bacteria isolated from the mangroves Avicennia germinans and Laguncularia racemosa
Publication from thesis:
  • None so far (tutor passed away before preparing a manuscript)
  • Alfonso Working

    Alfonso Davila purifying signal molecules from bacteria isolated from mangroves
    Name: Alfonso Davila Lule
    Degree: M. Sc.
    Years: 2004-2007
    Graduate school: The Center for Biological Research of the Northwest. La Paz, Mexico.
    Thesis: Effect of signal molecule (acile homoserine lactone) produce by Vibrio spp. LR6HC, bacteria isolated from the roots of black mangrove Avicennia germinans.
    Publication from thesis:
  • None so far (tutor passed away before preparing a manuscript)
  • Octavio Perez

    Octavio Perez evaluating an autotrophic microalgal culture before setting
    an experiment using Chlorella vulgaris jointly immobilized with Azospirillum
    brasilense
    under heterotrophic growth conditions (no available light, only nutrients).
    Name: Octavio Perez
    Degree: M.Sc.
    Years: 2006-2009
    Graduate school: The Center for Biological Research of the Northwest. La Paz, Mexico.
    Thesis: Biological nutrients removal from wastewater using microalgae-bacteria immobilized system under authotrophic, heterotrophic and mixotrophic conditions.
    Publications from thesis:
    1. Perez-Garcia, O., de-Bashan, L.E., Hernandez, J.-P., and Bashan, Y. 2010. Efficiency of growth and nutrient uptake from wastewater by heterotrophic, autotrophic, and mixotrophic cultivation of Chlorella vulgaris immobilized with Azospirillum brasilense. Journal of Phycology 46: 800–812
    2. Perez-Garcia, O., Escalante, F.M.E., de-Bashan L.E., and Bashan, Y. 2011. Heterotrophic cultures of microalgae: Metabolism and potential products. Water Research 45: 11-36. The definitive version is available at: http://www.elsevier.com
    3. Perez-Garcia, O., Bashan, Y., and Puente M.E. 2011. Organic carbon supplementation of sterilized municipal wastewater is essential for heterotrophic growth and removing ammonium by the microalga Chlorella vulgaris. Journal of Phycology 47: 190-199.

    Name: Adan Trejo Rangel
    Degree: M.Sc.
    Years: 2007-2010
    Graduate school: The Center for Biological Research of the Northwest. La Paz, Mexico.
    Thesis: The potential use of microbiological residues from biological wastewater treatment; their application as inoculant for improvement of eroded soils.
    Adan FISH azospirillum

    Root analysis by a molecular technique (FISH) using an image
    analyzer equipped with florescent microscope by Adan Trejo
    Publications from thesis:
    1. Trejo, A., de-Bashan, L. E., Hartmann, A., Hernandez, J.P., Rothballer, M., Schmid, M. and Bashan, Y. 2012. Recycling waste debris of immobilized microalgae and plant growth-promoting bacteria from wastewater treatment as a resource to improve fertility of eroded desert soil. Environmental and Experimental Botany 75 : 65-73

    2. Lopez, B.R., Bashan, Y., Trejo, A., and de-Bashan, L.E. 2013. Amendment of degraded desert soil with wastewater debris containing immobilized Chlorella sorokiniana and Azospirillum brasilense significantly modifies soil bacterial community structure, diversity, and richness. Biology and Fertility of Soils 49: 1053-1063.

    Publication from thesis:
    1. Covarrubias, S.A., de-Bashan, L.E., Moreno, M., and Bashan, Y. 2012. Alginate beads provide a beneficial physical barrier against native microorganisms in wastewater treated with immobilized bacteria and microalgae. Applied Microbiology and Biotechnology 93: 2669-2680
      The definitive version is available electronically on SpringerLink: http://www.springerlink.com/openurl.asp?genre=article&id=doi:10.1007/s00253-011-3585-8
    sergio covarrubias

    Sergio Covarrubias preparing new experiments to analysis the interaction of the microbial community of domestic wastewater with the microalga Chlorella vulgaris co-immobilized with the MGPB Azospirillum brasilense
    Name: Sergio Hernandez Covarrubias
    Degree: M.Sc.
    Years: 2009-2011
    Graduate school: The Center for Biological Research of the Northwest. La Paz, Mexico.
    Thesis: Immobilization of microorganisms for wastewater treatment in alginate beads provides them a beneficial physical barrier against the native organisms residing in wastewater

    Name: Ivonne Cruz Santander
    Degree: M.Sc.
    Years: 2010-2012
    Graduate school: The Center for Biological Research of the Northwest. La Paz, Mexico.
    Thesis: Evaluation in situ of the immobilized system Chlorella vulgaris-Azospirillum brasilense during tertiary wastewater treatment.
    ivonne cruz

    Ivonne Cruz measuring phosphorus and ammonium obtained from a photobioreactor and heterotrophic bioreactor treating wastewater with the microalgae Chlorella vulagris.
    Publication from thesis:
    1. Cruz, I., Bashan, Y., Hernandez-Carmona, G., and de-Bashan, L.E. 2013. Biological deterioration of alginate beads containing immobilized microalgae and bacteria during tertiary wastewater treatment. Applied Microbiology and Biotechnology 97: 9847-9858

    Publications from thesis:
    1. Meza, B., de-Bashan, L. E., and Bashan, Y. 2015 Involvement of indole-3-acetic acid produced by Azospirillum brasilense in accumulating intracellular ammonium in Chlorella vulgaris. Research in Microbiology 166: 72-83

    2. Meza, B., de-Bashan, L. E., Hernandez, J. P., and Bashan, Y. 2015 Accumulation of intra-cellular polyphosphate in Chlorella vulgaris cells is related to indole-3-acetic acid produced by Azospirillum brasilense. Research in Microbiology 166: 399-407

    beatriz meza Name: Beatriz Meza Marquez
    Degree: M.Sc.
    Years: 2010-2013
    Graduate school: The Center for Biological Research of the Northwest. La Paz, Mexico.
    Thesis: Effect of indole-3-acetic-acid-produced by Azospirillum brasilense on the enzymes of ammonium assimilation of Chlorella vulgaris under immobilization conditions.

    Name: Emmanuel Vidana Avila
    Degree: M.Sc.
    Years: 2011-2014
    Graduate school: The Center for Biological Research of the Northwest. La Paz, Mexico.
    Thesis: Effect of glycerol as carbon source in the immobilized system of microalgae-bacteria under heterotrophic conditions for removing ammonium and phosphate.
    Emmanuel Vidaña

    Edgar Amavizca

    Name: Edgar Amavizca Valdez
    Degree: M.Sc.
    Years: 2011-2014
    Graduate school: The Center for Biological Research of the Northwest. La Paz, Mexico.
    Thesis: Evaluation of remote effects of plant growth-promoting bacteria on the microalgae Chlorella vulgaris.

    Cristina Galaviz Name: Cristina Galaviz Bustamante
    Degree: M.Sc.
    Years: 2012-2015
    Graduate school: The Center for Biological Research of the Northwest. La Paz, Mexico.
    Thesis: Changes in soil fertility and in the bacterial community of the rhizosphere of mesquite after inoculation with plant growth-promoting bacteria aimed to restore eroded desert soils.

    Name: Edisa Garcia
    Degree: M.Sc.
    Years: 2012-2015
    Graduate school: The Center for Biological Research of the Northwest. La Paz, Mexico.
    Thesis: Study of the structure and physiological profile of the soil bacterial community in the mesquite (prosopis articulata) resource islands in relation with the plant diversity
    Edisa Garcia

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